General principles for selection of antibacterial agents for biological reagents
There are many types of antibacterial agents for biological reagents, such as acids, bases and their salts, neutral compounds, mercury compounds, and quaternary amine compounds. Preservatives have a killing effect on microbial propagules, but for spores they can't develop into propagules and gradually die. Different types of preservatives have different mechanisms of action. For example, alcohols can denature pathogenic microorganism proteins; phenylmethylbenzene and parabens can combine with pathogenic microorganism enzyme systems to compete for their coenzymes; cationic surfactants, etc. It is by reducing the surface tension to increase the permeability of the bacterial cell membrane.
A good biological agent bacteriostatic agent should have the following characteristics:
1. The addition of biological reagent bacteriostatic agent should be able to effectively inhibit the growth of one or more microorganisms that are most likely to be contaminated during the preparation, storage and use of the kit; the preservative should be strong against most bacteria, fungi and yeast Inhibition
2. Biological reagent bacteriostatic agent has sufficient solubility in the reagent to ensure that it has sufficient concentration in the reagent of two-phase or multi-phase system;
3. During the validity period of the kit, the biological reagent bacteriostatic agent should be very stable, and will not reduce its concentration due to chemical degradation or volatilization; the physical and chemical properties and antimicrobial properties of the biological reagent bacteriostatic agent itself should be stable, not susceptible to heat and sample pH The influence of the value, it will not decompose and become invalid during long-term storage, will not volatile, will not precipitate, and will not work with packaging materials;
4. Biological reagent bacteriostatic agent does not react with any components in the kit, and will not affect the detection effect of the kit or the effect of the bacteriostatic agent;
5. Low cost of use and easy to obtain.
